Ex situ bioremediation techniques are usually considered based on. Bioremediation can be used at the site of contamination in situ or on contamination removed from the original site ex situ. The project attained no further action after 30 months of operation of a combined in situ bioremediation and fixed. It is the process of protecting an endangered species, variety or breed, of plant or animal outside its natural habitat. Bioremediation technologies can be generally classified as in situ or ex situ. Project manager for an in situ bioremediation project in des moines, ia. In situ and ex situ are two methods used to describe different biological processes such as. The url for each document is listed for your convenience but may be. In situ bioremediation is carried out at the contaminated site itself, so there is no need for soil removal. The soil can, however, be used for landscaping purposes. There is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix. Ex situ bioremediation method for the treatment of groundwater. Bioremediation is a grouping of technologies that use microbiota typically, heterotrophic bacteria and fungi to degrade or transform hazardous contaminants to materials such as carbon dioxide, water, inorganic salts, microbial biomass, and other byproducts that may be less hazardous than the parent materials.
Mahdi 1biology department, college of science, university of basrah, basrah, iraq. One of the main advantages of exsitu bioremediation is that it requires less time than the in situ treatment. Suitability for bioremediation bioremediation is not a new concept and is being increasingly used as a relatively economical. Accepted for publication in environmental remediation and restoration of contaminated nuclear and norm sites, l. The detailed results of ex situ controlled aerobic bioremediation of ten sites with differing petroleum contamination and four in situ cases are reported and compared. In situ processes treat soils and ground water in place, without removal. To improve soil structure, mate rials such as gypsum or organic matter can be ap plied. Created using powtoon free sign up at create animated videos and animated presentations for free. In situ and ex situ bioremediation of radionuclides contaminated soils at nuclear and norm sites francis, a. Low soil permeability can impede movement of water, nutrients, and oxygen. Integration of bioremediation with other technologies the use of living microbes to transform undesirable. Section 2 of the report provides an overview of bioremediation technologies, including in situ and ex situ technologies, and provides examples of field use for three types of bioremediation technologies. In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site specific conditions, and efficacy when properly implemented.
It also suffers from costs associated with solid handling process e. In situ bioremediation technologies include bioventing, bio sparging, bio stimulation,liquid delivery systems and some composting methods. Nikolopoulou m, pasadakis n, norf h, kalogerakis n 20 enhanced ex situ bioremediation of crude oil contaminated beach sand by supplementation with nutrients and rhamnolipids. Pdf exsitu and insitu bioremediation strategies with special. Ex situ bioremediation of soil contaminated with crude oil by use of actinomycetes consortia for process bioaugmentation 1ahmed a.
The characteristics of bioremediation projects at superfund and other sites are described in section 3. In situ bioremediation involves treating the contaminated material at the site, while ex situ involves the removal of the contaminated material to be treated elsewhere. Ex situ bioremediation of soil contaminated with crude oil by use of actinomycetes consortia for process bioaugmentation. Ex situ bioremediation of soil contaminated with crude oil. Ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. Ex situ bioremediation technology includes most of disadvantages and limitations. The in situ remediation methods with microorganisms are based on natural attenuation, biostimulation, or bioaugmentation. This guideline applies only to the ex situ treatment of soils. In situ bioremediation in situ bioremediation is the clean up approach which directly involves the contact between microorganisms and the dissolved and sorbed contaminants for biotransformation bouwer and zehnder, 1993.
Ex situ bioremediation list of high impact articles. Microorganisms used to perform the function of bioremediation are known as bioremediators1. It can also disrupt the site and requires a good deal of space. In situ bioremediation environmental biotechnology.
Bioremediation has the advantage of using natural processes to clean up sites. The newly introduced phytoremediation treatment, involving plant uptakedegradation of contaminants, can be. The main advantage of ex situ treatment is that it generally requires shorter time periods than in situ treatment, and there is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix the soil. Bioremediation technologies for soil composting addition of moisture and nutrients, regular mixing for aeration biopiles exsitu aeration of soil bioventing insitu aeration of soil land treatment application of organic materials to natural soils followed by irrigation and tilling. Ex situ bioremediation involves extraction of contaminated media to other location for treatment. Bioremediation techniquesclassification based on site of. There are two types of bioremediation ex situ remove and treat, and in situ treat in place and can be performed on soil or underground water. Due to the ex situ techniques used to decontaminate the soil, much of the soil cannot be used as filling or agricultural material. The main advantage of ex situ treatment is that it generally requires shorter time periods, and there is more certainty about the uniformity of treatment because of the ability to monitor and continuously mix the groundwater. Ex situ conservation literally means, offsite conservation. Exsitu bioremediation is usually more successful than insitu bioremediation. Exsitu treatment technology is further divided into slurryphase bioremediation and solidphase. For these reasons, ex situ methods sometimes are impractical.
Due to in situ bioremediation taking place at the site of contamination there is a lessened risk of cross contamination as opposed to ex situ bioremediation where the polluted material is transported to other sites. The in situ bioremediation isb documents address the systematic characterization, evaluation, and appropriate design and testing of isb for biotreatable contaminants. Six pumping wells, two recharge wells and fifteen observation wells were installed for this study as a part of the ex situ biodegradation treatment plant. Performance criteria, which also determine the choice of ex situ bioremediation techniques, have been described philp and atlas 2005. Ex situ bioremediation of shrimp culture effluent using constructed microbial mats. Insitu and exsitu bioremediation technologies p1 duration.
Exsitu bioremediation of shrimp culture effluent using. In situ bioremediationthe use of microorganisms for onsite removal of contaminantsis potentially cheaper, faster, and safer than conventional cleanup methods. Ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at a place to facilitate microbial degradation. Exsitu anaerobic bioremediation exsitu bioremediation occurs after contaminated soils are excavated. Ex situ processes involve the removal of the contaminated media to a treatment area. A major advantage of ex situ bioremediation processes is that most of the decontaminated soil can be reused. Technologies can be generally classified as in situ or ex situ. Biological treatment has been a major component for many years in the. The strategies used for soil bioremediation can be through in situ and ex situ methods. Another advantage is the certainty of the control treatment due to the ability to uniformly screen, homogenize and mix the soil. Biopiles or windrows are an ex situ application of bioremediation where petroleum hydrocarbon impacted. Landfarming landfarming is a simple technique in which contaminated soil is excavated and spread over a prepared bed and periodically tilled until pollutants are.
Introduction to in situ bioremediation of groundwater. Here in this introductory chapter, bioremediation and bioremediation techniques are introduced. It is concluded, that bioremediation of petroleum contaminated soil is an effective, safe and economically feasible remediation technique for the remediation of such. Excavation and pumping also will occur for ex situ bioremediation. These documents are available in pdf format on the internet from the u. In situ in situ and ex situ ex situ bioremediation technologies for groundwater contaminated with chlorinated solvents michelle m. Or, rightclick on the link and save the document to your local drive. Potential advantages of in situ bioremediation methods include minimal. Fundamental principles of bioremediation an aid to. In the case of contaminated soil, sediments, and sludges, it.
Ex situ bioremediation ex situ bioremediation is only used when necessary because its expensive and damaging to the area, since the contaminated land is physically removed. Bioremediation an overview 165 water, and nutrients. Phytoremediation acknowledgements glossary bibliography biographical sketch summary a brief outline of the development of bioremediation technologies is presented. Examples of ex situ processes include land treatment and composting. But in situ bioremediation is also clouded in uncertainty, controversy, and mistrust. Because ex situ methods require excavation, they disrupt the landscape, expose contaminants, and require replacement of soils. Depending on the state of the contaminant in the step of bioremediation, ex situ. It can be expensive, mostly due to the cost of excavation and transportation of the soil. In situ bioremediation can also have lower costs and a higher rate of decontamination than ex situ bioremediation. It is removal and collection ofwaste at a place to facilitates biodegradation. Types of in situ bioremediation1 intrinsic in situ bioremediation. Potential advantages of bioremediation compared to other in situ methods.
It consists of placing the excavated materials into an above ground enclosure. Bioremediation as a branch of environmental biotechnology takes advantage of various living organisms including bacteria, fungi, algae, and plants in order to remediate the contaminated ecosystems. Ex situ bioremediation environmental biotechnology. See a citizens guide to excavation of contaminated soil epa 542f12007.
Biotransformation in the surface environment is a very complex process. An overview of conventional and innovative technologies. Water remediation methods are ex situ and consist of a system in which pumping contaminated water out from the water table is necessary. In situ bioremediation an overview sciencedirect topics. You may need a pdf reader to view some of the files on this page. The potential effects of sawdust, and mixture of cow and sheep dung to biostimulant autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile, made of soil polluted with petroleum waste 100kg. Bioremediation is the use of any organism metabolism to remove pollutants. Historical information on the development of bioremediation can be found in martin and gershuny, 1992. Various bioremediation techniques can be applied in situ or ex situ and under aerobic or. Low temperature ex situ bioremediation of hydrocarbon. Insitu and exsitu bioremediation technologies p1 youtube. Furthermore, in situ methods are less expensive and less manageable while the ex situ methods are expensive and manageable. Ex situ bioremediation treatment landfarming springerlink. Bioremediation an overview 163 are imported to a contaminated site to enhance degradation we have process known as bioaugmentation.
Pdf on sep 14, 2014, s raja and others published exsitu and insitu bioremediation strategies with special reference to marine and coastal environments. Emily majcher, duane graves, geosyntec consultants. Click on a document title to view it online and print. This section discusses in situ and ex situ bioremediation. Low temperature ex situ bioremediation of hydrocarbon contaminated soils vince parsons, b. Ex situ bioremediationexsitu conservation means literally, offsiteconservation. The main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the.
Pdf ex situ bioremediation of soil contaminated with. In this the contaminated soil is excavated and removed from the site as completely as possible. Exsitu biological treatment one of the main advantages of exsitu bioremediation is that it requires less time than the insitu treatment. Bioremediation as a sustainable alternative to chemicalphysical processes is an attractive solution for soil decontamination and renewal of polluted sites. These techniques involve excavating pollutants from polluted sites and subsequently transporting them to another site for treatment. For each one the principle of operation and recent.